How to prepare Acetocarmine staining solution?

Acetocarmine stain is commonly used for cytological preparations.

For the acetocarmine stain preparation:

       Boil 45% acetic acid in a flask and add 0.5 g of carmine powder to it. Mix well and continue heating for 10-15 minutes. After that allow it to cool and filter it. Before using it, add few drops of ferric chloride solution.