Safranin is used as a counterstain.
For Safranin stain preparation:
For Safranin stain preparation:
Dissolve 1 gm safranin in 100 ml alcohol (95%) and dilute to required concentration with distilled water. Store at room temperature.
Showing posts with label Staining techniques. Show all posts
Showing posts with label Staining techniques. Show all posts
How to prepare Acetocarmine staining solution?
Acetocarmine stain is commonly used for cytological preparations.
For the acetocarmine stain preparation:
For the acetocarmine stain preparation:
Boil 45% acetic acid in a flask and add 0.5 g of carmine powder to it. Mix well and continue heating for 10-15 minutes. After that allow it to cool and filter it. Before using it, add few drops of ferric chloride solution.
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